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- Title
Changes in Expression Level of OsHKT1;5 Alters Activity of Membrane Transporters Involved in K+ and Ca2+ Acquisition and Homeostasis in Salinized Rice Roots.
- Authors
Alnayef, Mohammad; Solis, Celymar; Shabala, Lana; Ogura, Takaaki; Chen, Zhonghua; Bose, Jayakumar; Maathuis, Frans J. M.; Venkataraman, Gayatri; Tanoi, Keitaro; Yu, Min; Zhou, Meixue; Horie, Tomoaki; Shabala, Sergey
- Abstract
In rice, the OsHKT1;5 gene has been reported to be a critical determinant of salt tolerance. This gene is harbored by the SKC1 locus, and its role was attributed to Na+ unloading from the xylem. No direct evidence, however, was provided in previous studies. Also, the reported function of SKC1 on the loading and delivery of K+ to the shoot remains to be explained. In this work, we used an electrophysiological approach to compare the kinetics of Na+ uptake by root xylem parenchyma cells using wild type (WT) and NIL(SKC1) plants. Our data showed that Na+ reabsorption was observed in WT, but not NIL(SKC1) plants, thus questioning the functional role of HKT1;5 as a transporter operating in the direct Na+ removal from the xylem. Instead, changes in the expression level of HKT1;5 altered the activity of membrane transporters involved in K+ and Ca2+ acquisition and homeostasis in the rice epidermis and stele, explaining the observed phenotype. We conclude that the role of HKT1;5 in plant salinity tolerance cannot be attributed to merely reducing Na+ concentration in the xylem sap but triggers a complex feedback regulation of activities of other transporters involved in the maintenance of plant ionic homeostasis and signaling under stress conditions.
- Subjects
MEMBRANE transport proteins; HOMEOSTASIS; RICE; PLANT maintenance; XYLEM; MONOAMINE transporters; PSYCHOLOGICAL feedback
- Publication
International Journal of Molecular Sciences, 2020, Vol 21, Issue 14, p4882
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms21144882