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- Title
Non-Cytotoxic Agarose/Hydroxyapatite Composite Scaffolds for Drug Release.
- Authors
Witzler, Markus; Ottensmeyer, Patrick Frank; Gericke, Martin; Heinze, Thomas; Tobiasch, Edda; Schulze, Margit
- Abstract
Healing of large bone defects requires implants or scaffolds that provide structural guidance for cell growth, differentiation, and vascularization. In the present work, an agarose-hydroxyapatite composite scaffold was developed that acts not only as a 3D matrix, but also as a release system. Hydroxyapatite (HA) was incorporated into the agarose gels in situ in various ratios by a simple procedure consisting of precipitation, cooling, washing, and drying. The resulting gels were characterized regarding composition, porosity, mechanical properties, and biocompatibility. A pure phase of carbonated HA was identified in the scaffolds, which had pore sizes of up to several hundred micrometers. Mechanical testing revealed elastic moduli of up to 2.8 MPa for lyophilized composites. MTT testing on Lw35human mesenchymal stem cells (hMSCs) and osteosarcoma MG-63 cells proved the biocompatibility of the scaffolds. Furthermore, scaffolds were loaded with model drug compounds for guided hMSC differentiation. Different release kinetic models were evaluated for adenosine 5′-triphosphate (ATP) and suramin, and data showed a sustained release behavior over four days.
- Subjects
TISSUE scaffolds; ADENOSINE triphosphate; AGAROSE; HYDROXYAPATITE; MESENCHYMAL stem cells; DOSAGE forms of drugs
- Publication
International Journal of Molecular Sciences, 2019, Vol 20, Issue 14, p3565
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms20143565