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- Title
American ginseng (Panax quinquefolius) administration does not affect performance of the Roche COBAS Ampliprep/Taqman HIV-1 RNA assay.
- Authors
Bakshi, Rahul P.; Brown, Todd T.; Simmons, Antoine; Chun-Su Yuan; Bauer, Brent A.; Sloan, Jeff A.; Andrade, Adriana
- Abstract
Background: Previous data indicate that purified components of ginseng can inhibit HIV reverse transcriptase in vitro, suggesting that ginseng components in plasma may interfere with HIV-1 RNA detection assays. Methods: Pre- and post-dose plasma from three volunteers dosed with 3000 mg American ginseng was spiked with HIV and analyzed by the Roche COBAS Ampliprep/Taqman v2.0 HIV-1 RNA assay. Results: Presence of American ginseng had no significant effect on measured HIV-1 RNA concentration. Variation within pre- and post-dose plasma pair was insignificant and within assay performance limits. Conclusion: Plasma from subjects dosed with 3000 mg American ginseng does not interfere with the Roche COBAS Ampliprep/Taqman v2.0 HIV-1 RNA assay. This implies that in vitro inhibition of HIV reverse transcriptase by American ginseng components is unlikely to be clinically relevant.
- Subjects
BIOLOGICAL assay; GINSENG; HIV; HIV-positive persons; RNA; NUCLEOTIDYLTRANSFERASES; IN vitro studies
- Publication
BMC Complementary & Alternative Medicine, 2014, Vol 14, p1
- ISSN
1472-6882
- Publication type
Article
- DOI
10.1186/1472-6882-14-427