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- Title
Toxicological evaluation of Terminalia paniculata bark extract and its protective effect against CCI<sub>4</sub>-induced liver injury in rodents.
- Authors
Talwar, Sahil; Jagani, Hitesh V.; Nayak, Pawan G.; Kumar, Nitesh; Kishore, Anoop; Bansal, Punit; Shenoy, Rekha R.; Nandakumar, Krishnadas
- Abstract
Background: Based on the reported antioxidant and anti-inflammatory potential of Terminalia paniculata, the bark aqueous extract (TPW) was investigated against liver damage. Methods: Intrinsic cytotoxicity was tested on normal human liver (Chang) cell lines, followed by acute and subchronic toxicity studies in mice. TPW was then evaluated against CCI4-induced liver toxicity in rats. Liver enzymes (AST, ALT, and ALP) and antioxidant markers were assessed. The effect of TPW on isolated hepatic cells, post-CCl4 administration, was assessed by isolated mitochondrial membrane staining. The actions of TPW on apoptotic pathway in CCI4-treated Chang cells were also elucidated. Results: TPW was found to be safe at all doses tested in both in vitro and in vivo toxicity studies. TPW (400 mg/kg, p.o.) significantly (*p <0.05) improved liver enzyme activity as compared to CCI4. Also, it improved antioxidant status (GSH, GST, MDA and total thiol) and preserved hepatic cell architecture. TPW pre-treatment significantly attenuated the levels of phospho-p53, p53, cleaved caspase-3, phospho-Bad, Bad and cleaved PARP in CCI4-treated Chang cells, improving the viability considerably. Conclusion: The findings support a protective role for Terminalia paniculata in pathologies involving oxidative stress.
- Subjects
MEDICAL botany; ANIMAL experimentation; ANTIOXIDANTS; LIVER; PROBABILITY theory; RATS; OXIDATIVE stress
- Publication
BMC Complementary & Alternative Medicine, 2013, Vol 13, Issue 1, p127
- ISSN
1472-6882
- Publication type
Article
- DOI
10.1186/1472-6882-13-127