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- Title
Localization of Per a 3 allergen in the gut and faecal pellets of the American cockroach ( Periplaneta americana).
- Authors
Wu, H.-Q.; Liu, Z.-G.; Gao, B.; Li, M.; Ran, P.-X.; Xing, M.
- Abstract
Cockroach-derived materials are known to be a major source of potent aeroallergens, causing allergic respiratory diseases such as asthma and allergic rhinitis. The aim of this study was to determine the localization of the major allergen, Per a 3 (Cr-PI), within the American cockroach ( Periplaneta americana), which might reveal the relative importance of excreted materials and nonexcreted cockroach components as allergen sources. American cockroaches ( P. americana) and their faecal pellets were embedded in paraffin, and serial sections were cut and collected on glass slides. After being stained with mouse polyclonal antiserum against Per a 3, the sections were incubated sequentially with biotin-labelled sheep antimouse immunoglobulin G (IgG) and a preformed fluorescent isothiocyanate (FITC)-avidin complex. Finally, the sections were mounted and examined under a fluorescent microscope. Examination of Per a 3 immunoreactivity on the sections of the American cockroaches ( P. americana) revealed that the midgut mucosa, gut contents and faecal pellets were all strongly labelled. Per a 3 immunoreactive products were not detected in any other internal organs of the American cockroaches. These results suggest that Per a 3 allergen might be synthesized in and secreted from the epithelia of the midgut mucosa and excreted from the body in the faecal pellets.
- Subjects
ALLERGENS; AMERICAN cockroach; COCKROACHES as carriers of disease; OCCUPATIONAL allergies; ASTHMA; ALLERGIC rhinitis; IMMUNOGLOBULIN G
- Publication
International Journal of Immunogenetics, 2007, Vol 34, Issue 5, p347
- ISSN
1744-3121
- Publication type
Article
- DOI
10.1111/j.1744-313X.2007.00697.x