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- Title
Exploring antibiotic resistant mechanism by microcalorimetry II.
- Authors
Yang, Xia; Feng, Lei; Xu, Kang-Zhen; Gao, Hui-Zhou; Jia, Chao; Liu, Cheng-Cheng; Xiao, Jian-Min; Zhai, Le; Zhou, Li-Sheng; Yang, Ke-Wu
- Abstract
In an effort to understand the reaction of antibiotic hydrolysis with B2 metallo-β-lactamases (MβLs), the thermodynamic parameters of imipenem hydrolysis catalyzed by metallo-β-lactamase ImiS from Aeromonas veronii bv. sobria were determined by microcalorimetric method. The values of activation free energy $$ \Updelta G_{ \ne }^{\theta } $$ are 86.400 ± 0.043, 87.543 ± 0.034, 88.772 ± 0.024, and 89.845 ± 0.035 kJ mol at 293.15, 298.15, 303.15, and 308.15 K, respectively, activation enthalpy $$ \Updelta H_{ \ne }^{\theta } $$ is 18.586 ± 0.009 kJ mol, activation entropy $$ \Updelta S_{ \ne }^{\theta } $$ is −231.34 ± 0.12 J mol K, apparent activation energy E is 21.084 kJ mol, and the reaction order is 1.5. The thermodynamic parameters reveal that the imipenem hydrolysis catalyzed by metallo-β-lactammase ImiS is an exothermic and spontaneous reaction.
- Subjects
ANTIBIOTICS; DRUG resistance; MICROCALORIMETRY; CHEMICAL reactions; THERMODYNAMICS; ACTIVATION energy; BETA lactamases
- Publication
Journal of Thermal Analysis & Calorimetry, 2012, Vol 110, Issue 2, p945
- ISSN
1388-6150
- Publication type
Article
- DOI
10.1007/s10973-011-1844-7