We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Groove structure of porous hydroxyapatite scaffolds (HAS) modulates immune environment via regulating macrophages and subsequently enhances osteogenesis.
- Authors
Li, Chenglong; Yang, Lu; Ren, Xiaohua; Lin, Mu; Jiang, Xiliang; Shen, Daonan; Xu, Taotao; Ren, Jing; Huang, Lijuan; Qing, Wei; Zheng, Jiajun; Mu, Yandong
- Abstract
Researches have revealed the vital roles of the generated immune environment via the response of immune cells growing on biomaterial surfaces in the bone healing process. HAS and novel constructed microgrooved patterns of HAS (HAS-G) are widely used as biocompatible ceramic, especially as a mimic of the natural bone matrix. However, it is unclear whether osteoimmune response induced by HAS and HAS-G affects the osteogenic differentiation of bone marrow stromal cells (BMSCs). RAW264.7 cells were seeded on different surface of materials and cytokines released by macrophages were detected by enzyme-linked immunosorbent assay. The cell viability and mitochondrial function of macrophages seeded on different surface of materials were detected. Then, the effects of modified inflammatory microenvironment by macrophages on osteogenesis of BMSCs were measured by performing ALP staining, Alizarin Red S staining, and western blot. We confirmed that HAS-G is more favorable for RAW cell attaching and subsequently regulated the expression and release of cytokines/chemokines. Decrease in interleukin-6 (IL-6) release was further confirmed for contributing significantly to improve mitochondrial function in RAW cells. HAS-G-conditioned medium promoted osteogenic differentiation in BMSCs and was reversed by IL-6 addition. Decrease in IL-6 contributes to downregulation of miR-214 and subsequently upregulated p38/JNK pathway, which is potentially contributes to osteogenic promotion by HAS-G. This study is the first report to reveal the effects of HAS-G on osteogenesis via immune response, which could lead to a new insight into novel material for the advantage of biomaterials for tissue engineering applications.
- Subjects
BONE growth; INTERLEUKIN-6; BIOMATERIALS; ECOLOGY
- Publication
Journal of Biological Inorganic Chemistry (JBIC), 2019, Vol 24, Issue 5, p733
- ISSN
0949-8257
- Publication type
Article
- DOI
10.1007/s00775-019-01687-w