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- Title
Preoperative vitamin C supplementation improves colorectal anastomotic healing and biochemical parameters in malnourished rats.
- Authors
Danielski, Lucineia; Walczewski, Eduardo; Jesus, Clovisa; Florentino, Drielly; Giustina, Amanda; Goldim, Mariana; Kanis, Luiz; Pereira, Gregório; Pereira, Volnei; Felisberto, Francine; Petronilho, Fabricia
- Abstract
Purposes: The objective of this study was to evaluate the effect of supplementation with vitamin C on intestinal anastomosis healing in malnourished rats. Methods: Male Wistar rats were divided into three groups: (1) sham, well-nourished rats that received vehicle; (2) FR+Veh, rats that were subjected to food restriction and received vehicle; and (3) FR+VC, rats that were subjected to food restriction and received vitamin C. Four days before surgery, the animals received vitamin C (100 mg/kg/day) via gavage and underwent colon resection with anastomosis in a single plane. The survival rate of rats was monitored until day 7 after surgery. Regarding anastomosis tissues, we examined intra-abdominal adhesion index, hydroxyproline content, collagen density, inflammatory parameters, and oxidative damage to proteins and lipids. Results: Malnutrition decreases body weight and increases mortality; the survival rate was 90 % in group 1, 60 % in group 2, and 80 % in group 3. Vitamin C was able to increase hydroxyproline concentration and density of collagen and decrease the intra-abdominal adhesion index, as well as the infiltration of neutrophils and oxidative damage to proteins in malnourished rats compared to group treated with vehicle. Conclusions: Preoperative vitamin C supplementation can improve the intestinal anastomosis healing, biochemical alterations, and prolong survival in rats subjected to food restriction.
- Subjects
PHYSIOLOGICAL effects of vitamin C; BUTENOLIDES; WATER-soluble vitamins; SURGICAL anastomosis; COLON (Anatomy)
- Publication
International Journal of Colorectal Disease, 2016, Vol 31, Issue 11, p1759
- ISSN
0179-1958
- Publication type
Article
- DOI
10.1007/s00384-016-2647-x