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- Title
Differential TCR gene usage between WC1 <sup> − </sup> and WC1 <sup> + </sup> ruminant γδ T cell subpopulations including those responding to bacterial antigen.
- Authors
Blumerman, Seth L.; Herzig, Carolyn T. A.; Rogers, Aric N.; Telfer, Janice C.; Baldwin, Cynthia L.
- Abstract
Ruminant γδ T cells are divided into subpopulations based on the presence or absence of WC1 co-receptors (scavenger-receptor-cysteine-rich family members uniquely expressed on γδ T cells). Evidence suggests WC1+ are inflammatory while WC1− are regulatory and that they also differ in their tissue distribution. Recently, this paradigm was refined further as cells that produce interferon-γ and proliferate to autologous antigens, leptospira antigens, or IL-12 were largely found within the WC1+ subpopulation that bears the WC1.1 antigenic epitope but not that bearing the WC1.2 epitope. Here, the T cell receptor gene expression by these different subpopulations (WC1−, WC1.1+, and WC1.2+) was compared using flow cytometrically-purified cells and reverse transcriptase-polymerase chain reaction (RT-PCR). The WC1− γδ T cells had transcripts for all 11 possible combinations of the TRG subgroup V and C genes while those in both WC1+ subpopulations were restricted to TRGV3–TRGC5 and TRGV7–TRGC5. In contrast, all three subpopulations expressed transcripts from all four known bovine TRDV genes. Further analysis of the WC1+ γδ T cells that proliferated in leptospira antigen-stimulated cultures indicated that they do not represent a unique subpopulation within the larger WC1+ population based on their TCR gene usage. Moreover, sequencing of 65 transcripts showed that their junctional regions were diverse as TRGJ5-1, TRGJ5-2, TRDJ1, and TRDJ3 were used, and CDR3s ranged from 9 to 24 amino acids. The restricted but shared γδ TCR gene usage for WC1.1 + , WC1.2+, and WC1+-antigen-responsive cells leaves open the possibility that the WC1 co-receptor is an important determining element in the activation process and subsequent response.
- Subjects
T cells; BACTERIAL antigens; CELL receptors; TISSUES; EPITOPES; GENE expression
- Publication
Immunogenetics, 2006, Vol 58, Issue 8, p680
- ISSN
0093-7711
- Publication type
Article
- DOI
10.1007/s00251-006-0122-5