We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Effects of ultra-marathon on circulating DNA and mRNA expression of pro- and anti-apoptotic genes in mononuclear cells.
- Authors
Atamaniuk, Johanna; Stuhlmeier, Karl M.; Vidotto, Claudia; Tschan, Harald; Dossenbach-Glaninger, Astrid; Mueller, Mathias M.
- Abstract
We investigated the effects of an ultra-marathon on cell-free plasma DNA as well as on mRNA expression of pro-apoptotic (Bax, Bad), anti-apoptotic (Bcl-2) and cell-protective (Hsp70, Hsp27 and Hsp32) genes in mononuclear blood cells (MNCs). Blood samples were drawn from 14 athletes before and immediately after 6-h run. In addition, blood samples were also collected and analyzed 2 and 24 h after the end of the run. Levels of plasma DNA were significantly increased immediately after the marathon (P < 0.001) and were still higher 2 h later (P < 0.005), but significantly lower than those immediately after the race (P < 0.05). Cell-free plasma DNA returned to pre-race levels 24 h after the run. mRNA expressions of Hsp70, Hsp32 and Bax significantly increased in MNCs after the race, whereas Hsp27 and Bad mRNA expression levels showed no significant changes. Bcl-2 expressions decreased immediately after the race (P < 0.001), but increased in the 24 h later (P < 0.05). We conclude that apoptotic ladders of cell-free DNA following exhaustive exercise originate from apoptotic cells and that not only skeletal muscle cells but also leukocytes contribute to this phenomenon.
- Subjects
MESSENGER RNA; DNA; BLOOD cells; GENES; APOPTOSIS
- Publication
European Journal of Applied Physiology, 2008, Vol 104, Issue 4, p711
- ISSN
1439-6319
- Publication type
journal article
- DOI
10.1007/s00421-008-0827-2