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- Title
Continuous degradation of maltose: improvement in stability and catalytic properties of maltase (α-glucosidase) through immobilization using agar-agar gel as a support.
- Authors
Nawaz, Muhammad; Karim, Asad; Aman, Afsheen; Marchetti, Roberta; Qader, Shah; Molinaro, Antonio
- Abstract
Maltose degrading enzyme was immobilized within agar-agar support via entrapment method due to its industrial utilization. The maximum immobilization efficiency (82.77 %) was achieved using 4.0 % agar-agar keeping the diameter of bead up to 3.0 mm. The matrix entrapment showed maximum catalytic activity at pH 7.0 and temperature 65 °C. Substrate saturation kinetics showed that the K of immobilized enzyme increased from 1.717 to 2.117 mM ml where as Vmax decreased from 8,411 to 7,450 U mlmin as compared to free enzyme. The immobilization significantly increased the stability of maltase against various temperatures and immobilized maltase retain 100 % of its original activity after 2 h at 50 °C, whereas the free maltase only showed 60 % residual activity under same condition. The reusability of entrapped maltase showed activity up to 12 cycles and retained 50 % of activity even after 5th cycle. Storage stability of agar entrapped maltase retain 73 % of its initial activity even after 2 months when stored at 30 °C while free enzyme showed only 37 % activity at same storage conditions. Graphical Abstract: [Figure not available: see fulltext.].
- Subjects
MALTOSE; GLUCOSIDASES; BIODEGRADATION; ENCAPSULATION (Catalysis); CATALYTIC activity; AGAR
- Publication
Bioprocess & Biosystems Engineering, 2015, Vol 38, Issue 4, p631
- ISSN
1615-7591
- Publication type
Article
- DOI
10.1007/s00449-014-1302-6