We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Sputum Microbiome Dynamics in Chronic Obstructive Pulmonary Disease Patients during an Exacerbation Event and Post-Stabilization.
- Authors
López Caro, Juan Carlos; Santibáñez, Miguel; García Rivero, Juan Luis; Villanueva, Manuel; Sainz, Jesús; González Astorqui, Pablo; Hierro, Margarita; Rodríguez Porres, Mariano; Paras Bravo, Paula; Mira, Alex; Rodriguez, Juan Carlos; Galiana, Antonio
- Abstract
Background: Chronic obstructive pulmonary disease (COPD) affects up to 65 million people worldwide, and COPD exacerbation causes tissue damage and subsequent loss of lung function. It is a multifactorial event in which respiratory infections are involved, but little is known about its dynamics. Objectives: The objective of our study was to determine the microbiome composition during an exacerbation event and post-stabilization. Methods: We conducted an observational analytical study of a cohort of 55 COPD patients in which 2 sputum samples (the first taken during an exacerbation event and the second during clinical post-stabilization) were submitted to 16s RNA ribosomal analysis by Illumina Miseq Next Generation Sequencing (NGS). The presence of respiratory viruses was also determined. Results: Our study found a stable microbiome composition in the post-stabilization sputum samples of COPD patients, and 4 additional microbiomes in samples taken during the exacerbation, 3 of which showed a marked dysbiosis by Haemophilus, Pseudomonas, and Serratia. The fourth exacerbation microbiome had a very similar composition to post-stabilization samples, but some pathogens such as Moraxella and respiratory viruses were also found. Conclusions: Our study reveals the main protagonists involved in lung microbiome dynamics during an exacerbation event and post-stabilization in COPD patients by NGS analysis.
- Subjects
RNA analysis; HUMAN microbiota; HAEMOPHILUS; LONGITUDINAL method; OBSTRUCTIVE lung diseases; SCIENTIFIC observation; PSEUDOMONAS; SERRATIA; SPUTUM; VIRUSES; DISEASE exacerbation; SEQUENCE analysis
- Publication
Respiration, 2019, Vol 98, Issue 5, p447
- ISSN
0025-7931
- Publication type
Article
- DOI
10.1159/000501988