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- Title
Fibronectin Immobilization using Water-soluble Carbodiimide on Poly-L-lactic Acid for Enhancing Initial Fibroblast Attachment.
- Authors
Nagai, Megumi; Hayakawa, Tohru; Makimura, Masaharu; Yoshinari, Masao
- Abstract
The aim of this study is to evaluate the influence of fibronectin immobilization on poly-L-lactic acid (PLA) films on the initial attachment of human gingival fibroblasts. Carboxylic acid groups are chemically introduced on the PLA films' surface by surface hydrolysis with 0.5 M NaOH. The contact angle of PLA surface with respect to double-distilled water decreases significantly after NaOH hydrolysis. X-ray photoelectron spectroscopy (XPS) also reveals significantly higher intensities of CC-O/CC-O after NaOH hydrolysis. Fibronectin is immobilized onto the hydrolyzed PLA surface through a condensation reaction between the carboxylic acid groups on the hydrolyzed PLA surface and the amino groups of fibronectin using water-soluble carbodiimide. XPS analysis shows that the fibronectin-immobilized PLA surface is enriched with nitrogen atoms. The immobilization of fibronectin significantly enhances the number of initially attached human gingival fibroblasts on the PLA surface. No obvious differences in morphology are noted between fibroblasts cultured on native PLA and on fibronectin-immobilized PLA. Fibronectin can be immobilized onto the PLA surface after NaOH hydrolysis and this is effective in enhancing the initial attachment of human gingival fibroblasts.
- Subjects
FIBRONECTINS; LACTIC acid; FIBROBLASTS; HYDROLYSIS; PHOTOELECTRON spectroscopy; CARBOXYLIC acids
- Publication
Journal of Biomaterials Applications, 2006, Vol 21, Issue 1, p33
- ISSN
0885-3282
- Publication type
Article
- DOI
10.1177/0885328206055316