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- Title
Comparison of Workflows for Milk Lipid Analysis: Phospholipids.
- Authors
Li, Cheng; Liu, Zhiqian; Marett, Leah; Pryce, Jennie; Rochfort, Simone
- Abstract
Milk is a rich source of lipids, with the major components being triglycerides (TAG) and phospholipids (mainly phosphatidylcholine (PC), sphingomyelin (SM), phosphatidylethanolamine (PE), phosphatidylserine (PS) and phosphatidylinositol (PI)). Liquid chromatography-mass spectrometry (LC-MS) is the predominant technique for lipid identification and quantification across all biological samples. While fatty acid (FA) composition of the major lipid classes of milk can be readily determined using tandem MS, elucidating the regio-distribution and double bond position of the FA remains difficult. Various workflows have been reported on the quantification of lipid species in biological samples in the past 20 years, but no standard or consensus methods are currently available for the quantification of milk phospholipids. This study will examine the influence of several common factors in lipid analysis workflow (including lipid extraction protocols, LC stationary phases, mobile phase buffers, gradient elution programmes, mass analyser resolution and isotope correction) on the quantification outcome of bovine milk phospholipids. The pros and cons of the current LC-MS methods as well as the critical problems to be solved will also be discussed.
- Subjects
LIPID analysis; PHOSPHOLIPIDS; PHOSPHATIDYLSERINES; GRADIENT elution (Chromatography); MILK; LIPIDS; MILKFAT; LIQUID chromatography-mass spectrometry
- Publication
Foods, 2023, Vol 12, Issue 1, p163
- ISSN
2304-8158
- Publication type
Article
- DOI
10.3390/foods12010163