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- Title
Antimicrobial and Antibiofilm Effect of ε-Polylysine against Salmonella Enteritidis, Listeria monocytogenes , and Escherichia coli in Tryptic Soy Broth and Chicken Juice.
- Authors
Lee, Do-Un; Park, Yeong Jin; Yu, Hwan Hee; Jung, Suk-Chae; Park, Jung-Hee; Lee, Dae-Hee; Lee, Na-Kyoung; Paik, Hyun-Dong
- Abstract
ε-Polylysine (ε-PL) is a safe food additive that is used in the food industry globally. This study evaluated the antimicrobial and antibiofilm activity of antibacterial peptides (ε-PL) against food poisoning pathogens detected in chicken (Salmonella Enteritidis, Listeria monocytogenes, and Escherichia coli). The results showed that minimum inhibitory concentrations (MICs) ranged between 0.031–1.0 mg/mL, although most bacterial groups (75%) showed MICs of 1.0 mg/mL. The reduction in the cell viability of pathogens due to ε-PL depended on the time and concentration, and 1/2 × MIC of ε-PL killed 99.99% of pathogens after 10 h of incubation. To confirm biofilm inhibition and degradation effects, crystal violet assay and confocal laser scanning microscopy (CLSM) were used. The biofilm formation rates of four bacterial groups (Salmonella, Listeria, E. coli, and multi-species bacteria) were 10.36%, 9.10%, 17.44%, and 21.37% at 1/2 × MIC of ε-PL, respectively. Additionally, when observed under a CLSM, ε-PL was found to induce biofilm destruction and bacterial cytotoxicity. These results demonstrated that ε-PL has the potential to be used as an antibiotic and antibiofilm material for chicken meat processing.
- Subjects
ESCHERICHIA coli; FOOD poisoning; PEPTIDE antibiotics; COOKING stocks; FOOD pathogens; ANTIBACTERIAL agents; SALMONELLA enteritidis; LISTERIA monocytogenes
- Publication
Foods, 2021, Vol 10, Issue 9, p2211
- ISSN
2304-8158
- Publication type
Article
- DOI
10.3390/foods10092211