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- Title
ANTIBACTERIAL EVALUATION OF ESSENTIAL OILS OF JUNIPERUS COMMUNIS, PINUS ROXBURGHII AND THUJA OCCIDENTALIS AGAINST ESCHERICHIA COLI.
- Authors
Singh, Ravikant; Pathak, Ashutosh; Dikshit, Anupam; Mishra, Rohit K.
- Abstract
The present investigation focused on the determination of antibacterial efficacy of essential oils obtained from leaves of three gymnosperms i.e. Juniperus communis L., Pinus roxburghii Sarg. andThuja occidentalis L. The oils were extracted from the leaves of aforementioned plant species using hydro-distillation method. The antibacterial activity of the extracted essential oils was evaluated against Escherichia coli (MTCC No. 723) using broth micro-dilution method recommended by Clinical Laboratory Standard Institute (CLSI) formerly (NCCLS). The Inhibition Concentration i.e. IC50 and Minimum Inhibition concentrations (MIC) using Spectrama x Plus384, of Molecular Devices Corporation, USA were recorded while Streptomycin as standard was taken. The IC50 value of J. communis L., P. roxburghii Sarg. and T. occidentalis L., were showed 0.010, 0.004 and 0.075 mg/ml respectively. The T. occidentalis L., was found most effective withtheir MIC 0.084 mg/ml while J. communis L., showed least effective with their MIC 0.390 mg/ml against E. coli. Hence, essential oil from leaves of gymnosperms exhibit great potential for the development of eco-friendly, nontoxic, cost-effective anti-bacterial formulations.
- Subjects
ANTIBACTERIAL agents; ESSENTIAL oils; JUNIPERUS communis; THUJA occidentalis; ESCHERICHIA coli
- Publication
Biochemical & Cellular Archives, 2018, Vol 18, Issue 1, p241
- ISSN
0972-5075
- Publication type
Article