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- Title
Increased mtDNA mutation frequency in oocytes causes epigenetic alterations and embryonic defects.
- Authors
Han, Longsen; Chen, Yujia; Li, Ling; Ren, Chao; Wang, Haichao; Wu, Xinghan; Ge, Juan; Shu, Wenjie; Chen, Minjian; Wang, Qiang
- Abstract
Mitochondria are essential for female reproductive processes, yet the function of mitochondrial DNA (mtDNA) mutation in oocytes remains elusive. By employing an mtDNA mutator (Polgm) mouse model, we found the fetal growth retardation and placental dysfunction in post-implantation embryos derived from Polgm oocytes. Remarkably, Polgm oocytes displayed the global loss of DNA methylation; following fertilization, zygotic genome experienced insufficient demethylation, along with dysregulation of gene expression. Spindle–chromosome exchange experiment revealed that cytoplasmic factors in Polgm oocytes are responsible for such a deficient epigenetic remodeling. Moreover, metabolomic profiling identified a significant reduction in the α-ketoglutarate (αKG) level in oocytes from Polgm mice. Importantly, αKG supplement restored both DNA methylation state and transcriptional activity in Polgm embryos, consequently preventing the developmental defects. Our findings uncover the important role of oocyte mtDNA mutation in controlling epigenetic reprogramming and gene expression during embryogenesis. αKG deserves further evaluation as a potential drug for treating mitochondrial dysfunction-related fertility decline.
- Subjects
MITOCHONDRIAL DNA; FETAL growth retardation; OVUM; EPIGENETICS; DNA methylation; FERTILITY decline
- Publication
National Science Review, 2022, Vol 9, Issue 10, p1
- ISSN
2095-5138
- Publication type
Article
- DOI
10.1093/nsr/nwac136