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- Title
大豆转录因子GmDofl.5 的克隆与非生物胁迫诱导表达.
- Authors
何佳琦; 翟莹; 张军; 邱爽; 李铭杨; 赵艳; 张梅娟; 马天意
- Abstract
Dof transcription factors play an important regulatory role in plant growth and development and response to abiotic stress . A soybean Dof transcription factor GmDojI . 5 with unknown function was cloned through RT-PCR, which was located on chromosome 15 of the soybean genome. The ORF of GmDefl . 5 was 540 bp, which encoded 179 amino acid residues with molecular weight of 20. 15 ku and isoelectric point of 9. 82. Prediction of protein sequence showed that GmDofl. 5 contained a typical Zf-Dof binding domain and a large number of phosphorylation sites. Phylogenetic analysis indicated that GmDofl. 5 of soybean was highly homologous to SsDof4 of Spatholobus suberectus. Quantitative real-time PCR showed that the expressions of GmDofI. 5 were induced by ABA, drought, salt, high temperature and cold stresses to different degrees. Especially, the response of GmDefl . 5 to high temperature was the most obvious.
- Publication
Acta Agriculturae Zhejiangensis, 2021, Vol 33, Issue 1, p1
- ISSN
1004-1524
- Publication type
Article
- DOI
10.3969/j.issn.1004-1524.2021.01.01