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- Title
Exploring Capillary Electrophoresis-Mass Spectrometry for the In-Depth Intact Native Analysis of Cysteine-Conjugated Antibody-Drug Conjugates.
- Authors
Füssl, Florian; Strasser, Lisa; Carillo, Sara; Bones, Jonathan
- Abstract
Recent years have seen dramatic technological developments, paving the way for the hyphenation of native, salt-based or salt-assisted chromatographic modes and capillary electrophoresis, which separate biomolecules according to size, charge or hydrophobicity, to mass spectrometry (MS). Such techniques can be employed to unravel the heterogeneity of glycoproteins while preserving higher order structure and non-covalent interactions. Therefore, these methods offer great potential for the comprehensive analysis of complex and labile biopharmaceuticals such as antibody-drug conjugates (ADCs). This article explores the potential of microchip capillary electrophoresis-mass spectrometry (CE-MS) for the characterization of intact biopharmaceuticals, and how this technique can effectively be employed to monitor essential quality attributes of interest.
- Subjects
ANTIBODY-drug conjugates; CAPILLARY electrophoresis; SPECTROMETRY; MASS spectrometry; BIOPHARMACEUTICS; BIOMOLECULES
- Publication
LC-GC Europe, 2020, Vol 33, Issue 10, p6
- ISSN
1471-6577
- Publication type
Article