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- Title
Synchrotron nano-FTIR spectroscopy for probing anticancer drugs at subcellular scale.
- Authors
de Carvalho, Luis A. E. Batista; Cinque, Gianfelice; de Carvalho, Ana L. M. Batista; Marques, Joana; Frogley, Mark D.; Vondracek, Hendrik; Marques, Maria Paula M.
- Abstract
The cellular response to cisplatin was assessed in human osteosarcoma cells, using synchrotron-based (SR) Fourier Transform InfraRed nanospectroscopy (nano-FTIR) at the MIRIAM beamline B22 of Diamond Light Source (UK). This label-free mapping method delivered simultaneous morphological and biochemical information on a subcellular level (i.e. 100 s nanometer or better). Based on specific spectral biomarkers, the main biochemical constituents affected by the drug were identified at distinct locations within the cell´s inner body. Cisplatin was shown to have a noteworthy effect on proteins, mostly within the cytoplasm. A clear drug impact on cellular lipids was also observed. Within current literature on s-SNOM, this nanospectroscopy work represents a first successful application in life sciences providing full fingerprint nano-FTIR spectra across intact human cancer cells.
- Subjects
UNITED Kingdom; ANTINEOPLASTIC agents; SYNCHROTRONS; NEAR-field microscopy; LIFE sciences; CISPLATIN
- Publication
Scientific Reports, 2024, Vol 14, Issue 1, p1
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/s41598-024-67386-y