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- Title
Small RNA Sequencing Reveals Differentially Expressed piRNAs Related to Hypoxia in Tibetan Chickens.
- Authors
Zengrong ZHANG; Xiaosong JIANG; Mohan QIU; Chunlin YU; Huarui DU; Qingyun LI; Han PENG; Xia XIONG; Xiaoyan SONG; Chenming HU; Jialei CHEN; Chaowu YANG
- Abstract
The relevance of genetic mechanism to the phenotype of hypoxic adaptation remains elusive. Tibetan chickens typically used to investigate the mechanism for the adaptation of hypoxia and the recognition of hypoxia-related piRNA remains an open issue. The purpose of this study was to illustrate whether the piRNAs were related to hypoxic adaptation. First of all, the differentially expressed piRNAs (DEpiRNAs) were identified through RNA sequencing between the Tibetan chickens and Daheng broilers. Subsequently, the target genes of DEpiRNAs were predicted and annotated by software. The network was constructed by Cytoscape. In our study, a total of 277 DEpiRNAs (33 down-regulated, 244 up-regulated) were identified in the Tibetan chickens compared with the Daheng broilers. All of the 277 DEpiRNAs predicted 36 658 targeted genes. Gene Ontology (GO) analysis showed that the target genes were significantly enriched in the biological process correlated with proliferation and apoptosis of cells, including cell cycle, mitochondrial outer membrane permeabilization, and positive regulation of stress-activated mitogen-activated protein kinase cascade. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis implicated that the DEpiRNAs were mainly involved in immune and metabolism, including natural killer cell-mediated cytotoxicity, toll-like receptor signaling pathway and fatty acid metabolism. Furthermore, a predicted network with four piRNAs acted on 11 pathways via interacting with 22 target genes, in which piR-gga-1368839 regulated metabolic pathways by acting on DHCR24. In conclusion, we determined the DEpiRNAs in the Tibetan chickens and found that these piRNAs were associated with metabolism, which may be favorable for researching the biological adaptation to hypoxic stress.
- Subjects
RNA sequencing; NON-coding RNA; BIOLOGICAL adaptation; MITOGEN-activated protein kinases; PHENOTYPIC plasticity; KILLER cell receptors
- Publication
Agricultural Biotechnology (2164-4993), 2022, Vol 11, Issue 4, p52
- ISSN
2164-4993
- Publication type
Article