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- Title
The expression of TUSC3 in Preeclampsia and the function in trophoblast cell.
- Authors
LI Meng-yong-wei; KANG Jin-yu; LIN Dan; SUN Fei; JIE Qiu-ling; MA Yan-lin
- Abstract
Objective: In this study, we aimed to explore the expression of TUSC3 in Preeclampsia and to research the potential function of TUSC3 in placental trophoblast cells. Methods: We collected 10 cases of normal placental tissues and preeclampsia placental tissues, respectively. These parturient received treatment at the First Affiliated Hospital of Hainan Medical University between June 1,2020, and December 31, 2022. The expression of TUSC3 in placenta was detected by immunohistochemistry. The effect of TUSC3 on the migration and invasion of HTR8/SVneo cells was analyzed by migration assay and Transwell assay. Results: The expression of TUSC3 was slightly increased in placental villis in preeclampsia. Immunohistochemistry and qRT-PCR were used to detect the expression of TUSC3 protein and mRNA in placental tissues. TUSC3 was markedly upregulated in PE placental tissues (P<0.01). The results of migration assay and Transwell assay showed that the migration rate and the number of invasive cells were significantly decreased in HTR8 overexpressing TUSC3(P<0.01). Conclusions: TUSC3 was markedly increased in PE placental tissues and inhibited trophoblast cells migration and invasion.
- Subjects
PREECLAMPSIA; CELL physiology; PLACENTAL growth factor; PREGNANCY proteins; CELL migration; PLACENTA
- Publication
Journal of Hainan Medical University, 2023, Vol 29, Issue 18, p30
- ISSN
1007-1237
- Publication type
Article