We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
p51/p63 inhibits ultraviolet B-induced apoptosis via Akt activation.
- Authors
Ogawa, E.; Okuyama, R.; Ikawa, S.; Nagoshi, H.; Egawa, T.; Kurihara, A.; Yabuki, M.; Tagami, H.; Obinata, M.; Aiba, S.
- Abstract
The epidermis must be protected against excess apoptotic cell death in response to ultraviolet-B (UV-B) irradiation. p53 is known to be critical for this protection. Although the p53 family member ΔNp51B/ΔNp63α (an N terminal-deleted form of p51/p63) is abundantly expressed in keratinocytes, its contribution to UV-B-dependent apoptosis is largely unknown. We found that, after a transient increase, ΔNp51B is downregulated in UV-B-irradiated keratinocytes undergoing apoptosis, whereas p53 is upregulated with delayed kinetics. Furthermore, the reduction of ΔNp51B by small interfering RNAs augmented UV-B-dependent apoptosis in keratinocytes, indicating that ΔNp51B blocks keratinocyte apoptosis. Although the exogenous expression of ΔNp51B in keratinocytes did not further block the UV-B-dependent apoptosis, to our surprise the expression of TAp51B (an isoform with a full NH2-terminal transactivation domain that is structurally and functionally similar to p53) decreased apoptosis significantly. The blockade of keratinocyte apoptosis by the p51 was dependent on the phosphorylation of Akt, resulting in the activation of a survival pathway. Thus, in addition to its indispensable roles in epithelial development, p51 acts in adult cells to protect the epidermis against UV-B irradiation by preventing excess depletion of keratinocytes.Oncogene (2008) 27, 848–856; doi:10.1038/sj.onc.1210682; published online 23 July 2007
- Subjects
ULTRAVIOLET radiation; CELL death; APOPTOSIS; KERATINOCYTES; EPIDERMIS
- Publication
Oncogene, 2008, Vol 27, Issue 6, p848
- ISSN
0950-9232
- Publication type
Article
- DOI
10.1038/sj.onc.1210682