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- Title
Optimization of gluco-amylase production from <italic>Aspergillus</italic> spp. for its use in saccharification of liquefied corn starch.
- Authors
Jain, Deepali; Katyal, Priya
- Abstract
Fungal gluco-amylase is required for the production of sugars from starchy substrates. Commercially available fungal gluco-amylase is quite costly which makes the process uneconomical. This study was undertaken to standardize physico-chemical parameters for optimum production of gluco-amylases from <italic>Aspergillus</italic> spp. Two fungal cultures, i.e., <italic>Aspergillus niger</italic> and <italic>Aspergillus terreus</italic>, were compared for gluco-amylase activity both under stationary and shake flask conditions. Among two fungal cultures, maximum gluco-amylase activity was shown by <italic>A. niger</italic> (243.09 U/ml) under stationary conditions as compared to <italic>A. terreus</italic> (126.34 U/ml). Gluco-amylase activity of <italic>A. niger</italic> increases by 42.48% from 243.09 to 346.35 U/ml after optimization using response surface methodology, whereby a substrate concentration of 7%, yeast extract 0.25%, temperature 32.5 °C and pH 5.5 were found to be optimum for gluco-amylase production. Crude enzyme was compared with commercial enzyme and it was found that when 500 U of Glucoamylase ex. <italic>Rhizopus</italic> were inoculated into starch-supplemented minimal media (SSMM) liquefied using 2 g of fungal diastase, it increases the reducing sugar concentration from 2.19 to 21.15 mg/ml and a saccharification efficiency of 77.7% was achieved, whereas 1.5 ml of crude enzyme (extracted from <italic>A. niger</italic>) was able to produce 14.46 mg/ml of reducing sugars with a saccharification efficiency of 53.2%.
- Subjects
GLUCOAMYLASE; ASPERGILLUS terreus; ASPERGILLUS niger; CORNSTARCH; RESPONSE surfaces (Statistics)
- Publication
3 Biotech, 2018, Vol 8, Issue 2, p0
- ISSN
2190-572X
- Publication type
Article
- DOI
10.1007/s13205-018-1131-4