We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Expression of N-acetylglucosaminyltransferase V in the subserosal layer correlates with postsurgical survival of pathological tumor stage 2 carcinoma of the gallbladder.
- Authors
Onuki, Kenichiro; Sugiyama, Hiroaki; Ishige, Kazunori; Kawamoto, Toru; Ota, Takehiro; Ariizumi, Shunichi; Yamato, Masayuki; Kadota, Shinichi; Takeuchi, Kaoru; Ishikawa, Akiko; Onodera, Masafumi; Onizawa, Kojiro; Yamamoto, Masakazu; Miyoshi, Eiji; Shoda, Junichi
- Abstract
Background: N-Acetylglucosaminyltransferase V (GnT-V), an enzyme that catalyzes the β1-6 branching of N-acetylglucosamine on asparagine-linked oligosaccharides of cellular proteins, enhances the malignant behaviors of carcinoma cells in experimental models. The aim of this study was to determine clinical significance of GnT-V expression in human pT gallbladder carcinoma with simple in vitro experiments. Methods: Ninety patients with pT gallbladder carcinoma were included for this study. The in vitro and in vivo biological effects of GnT-V were investigated using gallbladder carcinoma cells with variable GnT-V expression levels induced by a small interfering RNA. Results: Of the 90 cases, 57 showed positive staining and the remaining 33 demonstrated negative staining, the subcellular localization in the 57 cases was classified into the granular-type in 31 cases and the diffuse-type in 26 cases. In 76 cases with curative resection, postsurgical survival was significantly poorer in those showing positive staining than in those showing negative staining ( P = 0.028). In all of the 76 cases, postsurgical recurrence was significantly more frequent in those showing diffuse-type localization than in those showing negative staining. Experimental analyses demonstrated that the down-regulation of GnT-V expression in gallbladder carcinoma cells induced suppression of cell growth in vitro. The expression levels of GnT-V in the cells were highly correlated with the rapid in vivo growth coupled with the enhanced angiogenesis, and the tendency to form liver metastasis. Conclusions: GnT-V expression in the subserosal layer of pT gallbladder carcinoma is correlated with the aggressiveness of the disease.
- Subjects
GENE expression; N-acetylglucosaminyltransferase regulation; TUMOR surgery; GALLBLADDER cancer; ASPARAGINE; OLIGOSACCHARIDES; CANCER cells
- Publication
Journal of Gastroenterology, 2014, Vol 49, Issue 4, p702
- ISSN
0944-1174
- Publication type
Article
- DOI
10.1007/s00535-013-0813-2