We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Expression of CCL5 is induced by polyinosinic : polycytidylic acid in cultured hCMEC/D3 human brain microvascular endothelial cells.
- Authors
Arai, Akine; Yoshida, Hidemi; Hayakari, Ryo; Matsumiya, Tomoh; Kawaguchi, Shogo; Seya, Kazuhiko; Tanaka, Hiroshi; Imaizumi, Tadaatsu
- Abstract
Objective Brain microvascular endothelial cells are one of the cell types that form the blood-brain barrier, and play an important role in the defense system of the brain. Toll-like receptor 3 ( TLR3) is a pattern-recognition receptor against double-stranded RNA, and TLR3 signaling is important in antiviral innate immune reactions. However, TLR3 signaling in brain microvascular endothelial cells is not well understood. We aimed to investigate the role of TLR3 signaling in chemokine CCL5 production in human brain microvascular endothelial cells. Methods The hCMEC/D3 human brain microvascular endothelial cells were cultured, and treated with an authentic TLR3 agonist polyinosinic : polycytidylic acid. The expression of CCL5 was examined using quantitative real-time reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay. Involvement of TLR3, interferon ( IFN)-β, or IFN-λ1, IFN-regulatory factor 3 or nuclear factor-κB p65 in this reaction was examined using RNA interference. Translocation of p65 into the nucleus was examined using immunofluorescence staining. Results Treatment of cells with polyinosinic : polycytidylic acid induced the expression of CCL5, IFN-β and IFN-λ1, and also the translocation of p65 into the nucleus. Knockdown of TLR3, IFN-regulatory factor 3 or p65 inhibited the induction of these molecules, while knockdown of neither IFN-β nor IFN-λ1 affected the expression of CCL5. Conclusions TLR3 activation by polyinosinic : polycytidylic acid induces the expression of CCL5 in cultured hCMEC/D3 cells, and IFN-regulatory factor 3 and p65 are involved in this reaction. CCL5 induced by TLR3 signaling in brain microvascular endothelial cells might contribute to antiviral protective reactions and/or detrimental responses associated with viral infection in the brain.
- Subjects
ENDOTHELIAL cells; BLOOD-brain barrier; TOLL-like receptors; CHEMOKINES; REVERSE transcriptase polymerase chain reaction; POLYMERASE chain reaction
- Publication
Clinical & Experimental Neuroimmunology, 2017, Vol 8, Issue 4, p331
- ISSN
1759-1961
- Publication type
Article
- DOI
10.1111/cen3.12416