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- Title
MSTN Regulatory Network in Mongolian Horse Muscle Satellite Cells Revealed with miRNA Interference Technologies.
- Authors
Budsuren, Undarmaa; Ulaangerel, Tseweendolmaa; Shen, Yingchao; Liu, Guiqin; Davshilt, Toli; Yi, Minna; Bold, Demuul; Zhang, Xinzhuang; Bai, Dongyi; Dorjgotov, Dulguun; Davaakhuu, Gantulga; Jambal, Tuyatsetseg; Li, Bei; Du, Ming; Dugarjav, Manglai; Bou, Gerelchimeg
- Abstract
Myostatin (MSTN), a member of the transforming growth factor-β superfamily, inhibits the activation of muscle satellite cells. However, the role and regulatory network of MSTN in equine muscle cells are not well understood yet. We discovered that MSTN knockdown significantly reduces the proliferation rate of equine muscle satellite cells. In addition, after the RNA sequencing of equine satellite cells transfected with MSTN-interference plasmid and control plasmid, an analysis of the differentially expressed genes was carried out. It was revealed that MSTN regulatory networks mainly involve genes related to muscle function and cell-cycle regulation, and signaling pathways, such as Notch, MAPK, and WNT. Subsequent real-time PCR in equine satellite cells and immunohistochemistry on newborn and adult muscle also verified the MSTN regulatory network found in RNA sequencing analysis. The results of this study provide new insight into the regulatory mechanism of equine MSTN.
- Subjects
SATELLITE cells; MUSCLE cells; RNA sequencing; MICRORNA; HORSES; NOTCH genes; SOLEUS muscle; CELL cycle regulation
- Publication
Genes, 2022, Vol 13, Issue 10, pN.PAG
- ISSN
2073-4425
- Publication type
Article
- DOI
10.3390/genes13101836