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- Title
PHYTOCHEMICAL INVESTIGATION AND IN-VITRO IMMUNOMODULATORY STUDY OF ASPARAGUS RACEMOSUS WILLD ROOTS EXTRACTS.
- Authors
Parameshwara, C.; Jalalpure, Sunil S.; Jagwani, Satveer; Kumar, Nagraj N.; Shah, Biren
- Abstract
Asparagus racemosus Willd, commonly known as shatavari. It is an important medicinal plant found in tropical and subtropical areas in India. Various parts of the plant were traditionally used by Ayurvedic practitioners for inflammation, hepatic, neurological and certain infectious diseases. In the present study, the root of this plant was subjected to extraction with methanol followed by fractionation with solvents of increasing polarity such as petroleum ether, chloroform, ethyl acetate and methanol using a soxhlet extractor. Later phytochemical screening of crude methanolic extract and its fractions was done to identify active chemicals and studied further their in vitro immunomodulatory activity by using the nitroblue tetrazolium test, phagocytosis of killed Candida albicans, Candidacidal assay and neutrophil locomotion & chemotaxis. The extract and all the fractions were tested at concentrations viz. 5μg/ml, 10μg/ml, 25μg/ml, 50μg/ml and 100μg/ml respectively. Compare to other fractions, the chloroform fraction at high concentration showed predominantly significant (p<0.01) activity on in vitro human neutrophils in all tests, which are comparable to standard (in nitroblue tetrazolium test and neutrophil locomotion & chemotaxis) at different concentration indicating the possible immunostimulating effect. The present study reveals that, the presence of important phytoconstituents holds promise immunomodulatory activity in these fractions.
- Subjects
IMMUNOMODULATORS; ASPARAGUS; MEDICINAL plants; PLANT extracts; IN vitro studies; PHAGOCYTOSIS; COMMUNICABLE diseases; NITROBLUE tetrazolium
- Publication
Pharma Science Monitor, 2015, Vol 6, Issue 1, p292
- ISSN
0976-9242
- Publication type
Article