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- Title
PUMILIO1 Links Epilepsy to Spinocerebellar Ataxia.
- Authors
Lyman, Kyle A.; Chetkovich, Dane M.
- Abstract
A Mild PUM1 Mutation Is Associated With Adult-Onset Ataxia, Whereas Haploinsufficiency Causes Developmental Delay and Seizures Gennarino VA, Palmer EE, McDonell LM, et al. Cell. 2018;172(5):924-936.e11. doi:10.1016/j.cell.2018.02.006. Certain mutations can cause proteins to accumulate in neurons, leading to neurodegeneration. We recently showed, however, that upregulation of a wild-type protein, Ataxin1, caused by haploinsufficiency of its repressor, the RNA-binding protein Pumilio1 (PUM1), also causes neurodegeneration in mice. We therefore searched for human patients with PUM1 mutations. We identified 11 individuals with either PUM1 deletions or de novo missense variants who suffer a developmental syndrome (PUM1-associated developmental disability, ataxia, and seizure). We also identified a milder missense mutation in a family with adult-onset ataxia with incomplete penetrance (PUM1-related cerebellar ataxia). Studies in patient-derived cells revealed that the missense mutations reduced PUM1 protein levels by ∼25% in the adult-onset cases and by ∼50% in the infantile-onset cases; levels of known PUM1 targets increased accordingly. Changes in protein levels thus track with phenotypic severity, and identifying posttranscriptional modulators of protein expression should identify new candidate disease genes.
- Subjects
RNA-binding proteins; CEREBELLAR ataxia; SPINOCEREBELLAR ataxia; MISSENSE mutation; EPILEPSY; DEVELOPMENTAL disabilities; CEREBELLUM degeneration
- Publication
Epilepsy Currents, 2019, Vol 19, Issue 2, p122
- ISSN
1535-7597
- Publication type
Article
- DOI
10.1177/1535759719835354