We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
A High-Throughput Platform for Formulating and Screening Multifunctional Nanoparticles Capable of Simultaneous Delivery of Genes and Transcription Factors.
- Authors
Liu, Yang; Du, Juanjuan; Choi, Jin‐sil; Chen, Kuan‐Ju; Hou, Shuang; Yan, Ming; Lin, Wei‐Yu; Chen, Kevin Sean; Ro, Tracy; Lipshutz, Gerald S.; Wu, Lily; Shi, Linqi; Lu, Yunfeng; Tseng, Hsian‐Rong; Wang, Hao
- Abstract
Simultaneous delivery of multiple genes and proteins (e.g., transcription factors; TFs) is an emerging issue surrounding therapeutic research due to their ability to regulate cellular circuitry. Current gene and protein delivery strategies, however, are based on slow batch synthesis, which is ineffective, poorly controlled, and incapable of simultaneous delivery of both genes and proteins with synergistic functions. Consequently, advances in this field have been limited to in vitro studies. Here, by integrating microfluidic technologies with a supramolecular synthetic strategy, we present a high-throughput approach for formulating and screening multifunctional supramolecular nanoparticles (MFSNPs) self-assembled from a collection of functional modules to achieve simultaneous delivery of one gene and TF with unprecedented efficiency both in vitro and in vivo. We envision that this new approach could open a new avenue for immunotherapy, stem cell reprogramming, and other therapeutic applications.
- Subjects
NANOPARTICLES; TRANSCRIPTION factors; NEURAL circuitry; CHEMICAL synthesis; MICROFLUIDICS
- Publication
Angewandte Chemie International Edition, 2016, Vol 55, Issue 1, p169
- ISSN
1433-7851
- Publication type
Article
- DOI
10.1002/anie.201507546