We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
IN VITRO SCREENING OF CIRCIUM ARVENSE FOR POTENTIAL ANTIBACTERIAL AND ANTIFUNGAL ACTIVITIES.
- Authors
KHAN, ARIFULLAH; AMIN, ADNAN; KHAN, MUHAMMAD AYAZ; ALI, IRSHAD
- Abstract
The antimicrobial activities of Circium arvense extracts were evaluated against two human gram positive (Staphylococcus aureus, Micrococcus luteus) and four gram negative pathogen (Escherichia colt, pseudomonas aeruginosa, Enterobacter, Klebsiella pneumoniae). The Methanol extracts of Circium arvense were used to obtain various fractions (X1) n-hexane, (X2) chloroform, (X3) ethyl acetate and (X4) n-butanol. The agar well diffusion assay and agar dilution susceptibility testing were carried out to determine the zone of inhibitions and the minimum inhibitory concentration respectively. Antifungal activity was employed by agar well diffusion method. The Ampicilline, Ofloxacine and ltraconazole were used as standard agents. Almost all fractions exhibited more or less antimicrobial activity. The X2 (Chloroform) fraction was reported as highly active fraction with good antimicrobial activity MIC 0.312 mg/ml against staphylococcus areous. The MBC values of fraction X2 (chloroform) was 2 times the MIC. Similarly all fractions exhibited good inhibitory activities against Aspergillus niger. The fraction X1(n-hexane) was found highly active 6(±0.11), followed by X2 (chloform) fraction 5 (±0.17). Present study provides a base to explore the antimicrobial potential of Circium arvense. It is concluded that the Circium arvense having potential antibacterial and antifungal activities, irrespective of fact that the MIC values reported were high. Further investigations are recommended to exploit the hidden medicinal value of plant.
- Subjects
MEDICAL screening; CANADA thistle; ANTIBACTERIAL agents; ANTIFUNGAL agents; STAPHYLOCOCCUS aureus; GRAM-positive bacteria; MICROCOCCUS luteus; KLEBSIELLA pneumoniae; ENZYME inhibitors
- Publication
Pakistan Journal of Pharmaceutical Sciences, 2011, Vol 24, Issue 4, p519
- ISSN
1011-601X
- Publication type
Article