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- Title
Advancing virus-induced gene silencing in sunflower: key factors of VIGS spreading and a novel simple protocol.
- Authors
Mardini, Majd; Kazancev, Mikhail; Ivoilova, Elina; Utkina, Victoria; Vlasova, Anastasia; Demurin, Yakov; Soloviev, Alexander; Kirov, Ilya
- Abstract
Virus-Induced Gene Silencing (VIGS) is a versatile tool in plant science, yet its application to non-model species like sunflower demands extensive optimization due to transformation challenges. In this study, we aimed to elucidate the factors that significantly affect the efficiency of Agrobacterium-VIGS in sunflowers. After testing a number of approaches, we concluded that the seed vacuum technique followed by 6 h of co-cultivation produced the most efficient VIGS results. Genotype-dependency analysis revealed varying infection percentages (62–91%) and silencing symptom spreading in different sunflower genotypes. Additionally, we explored the mobility of tobacco rattle virus (TRV) and phenotypic silencing manifestation (photo-bleaching) across different tissues and regions of VIGS-infected sunflower plants. We showed the presence of TRV is not necessarily limited to tissues with observable silencing events. Finally, time-lapse observation demonstrated a more active spreading of the photo-bleached spots in young tissues compared to mature ones. This study not only offers a robust VIGS protocol for sunflowers but also provides valuable insights into genotype-dependent responses and the dynamic nature of silencing events, shedding light on TRV mobility across different plant tissues.
- Subjects
COMMON sunflower; BOTANY; GENE silencing; PLANT cells &; tissues; SUNFLOWERS
- Publication
Plant Methods, 2024, Vol 20, Issue 1, p1
- ISSN
1746-4811
- Publication type
Article
- DOI
10.1186/s13007-024-01241-z