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- Title
A mutation in Arabidopsis SAL1 alters its in vitro activity against IP<sub>3</sub> and delays developmental leaf senescence in association with lower ROS levels.
- Authors
Shirzadian-Khorramabad, Reza; Moazzenzadeh, Taghi; Sajedi, Reza H.; Jing, Hai-Chun; Hille, Jacques; Dijkwel, Paul P.
- Abstract
Key message: Our manuscript is the first to find a link between activity of SAL1/OLD101 against IP3 and plant leaf senescence regulation and ROS levels assigning a potential biological role for IP3. Leaf senescence is a genetically programmed process that limits the longevity of a leaf. We identified and analyzed the recessive Arabidopsis stay-green mutation onset of leaf death 101 (old101). Developmental leaf longevity is extended in old101 plants, which coincided with higher peroxidase activity and decreased H2O2 levels in young 10-day-old, but not 25-day-old plants. The old101 phenotype is caused by a point mutation in SAL1, which encodes a bifunctional enzyme with inositol polyphosphate-1-phosphatase and 3′ (2′), 5′-bisphosphate nucleotidase activity. SAL1 activity is highly specific for its substrates 3-polyadenosine 5-phosphate (PAP) and inositol 1, 4, 5-trisphosphate (IP3), where it removes the 1-phosphate group from the IP3 second messenger. The in vitro activity of recombinant old101 protein against its substrate IP3 was 2.5-fold lower than that of wild type SAL1 protein. However, the in vitro activity of recombinant old101 mutant protein against PAP remained the same as that of the wild type SAL1 protein. The results open the possibility that the activity of SAL1 against IP3 may affect the redox balance of young seedlings and that this delays the onset of leaf senescence.
- Subjects
DEVELOPMENTAL delay; MUTANT proteins; ARABIDOPSIS; RECOMBINANT proteins; GENETIC mutation
- Publication
Plant Molecular Biology, 2022, Vol 108, Issue 6, p549
- ISSN
0167-4412
- Publication type
Article
- DOI
10.1007/s11103-022-01245-0