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- Title
Impact of splenectomy on circulating microparticles in patients with sickle cell anemia.
- Authors
Boulassel, Mohamed‐Rachid; Al‐Rubkhi, Khoula; Al‐Qasabi, Jamal; El‐Ghamry, Islam; Khan, Hammad; Panjwani, Vinodh; Qureshi, Rizwan Nabi; Al‐Mamari, Sahimah; Al‐Qarni, Zahra; Al‐Kindi, Mohamed; Al‐Nabhani, Abdulrahman; Al‐Gharibi, Khalaf; Wali, Yasser; Pathare, Anil
- Abstract
Introduction: Circulating microparticles (MP) are being described as potential biomarkers for disease activity in a variety of conditions including sickle cell anemia (SCA). However, relatively little is known about the influence of spleen status on MP levels in patients with SCA. Methods: Using a prospective study design we characterize circulating MP in 144 patients with SCA in steady state by assessing their cellular origin and their relationships to spleen status defined by clinical and imaging findings. In addition, MP levels were studied according to demographic characteristics, clinical status, treatment modalities, and other hematological and biochemical parameters. Absolute plasma concentrations of MP were determined by flow cytometry. Results: Patients with SCA displayed a 10‐fold increase in levels of MP derived from red blood cell (RBC) and platelets (PLT) when compared to their healthy counterparts (p < 0.0001). Splenectomized patients with SCA have more pronounced levels of MPRBC and MPPLT, and remained elevated after several weeks of follow‐up. Levels of MP were not significantly associated with spleen removal procedures, age, gender, clinical severity score, hydroxyurea therapy, hemoglobin F, and co‐existence of glucose‐6‐phosphate dehydrogenase deficiency. Conclusion: Collectively, these results suggest that splenectomy affects circulating levels of MP regardless of the known SCA modifiers and correlates.
- Subjects
DRUG therapy for sickle cell anemia; SPLENECTOMY; BIOMARKERS; FLOW cytometry; BLOOD platelets; AGE distribution; HYDROXYUREA; SURGERY; PATIENTS; SEVERITY of illness index; COMPARATIVE studies; INBORN errors of carbohydrate metabolism; RESEARCH funding; ERYTHROCYTES; SOCIODEMOGRAPHIC factors; SICKLE cell anemia; EXTRACELLULAR vesicles; LONGITUDINAL method; FETAL hemoglobin; BLOOD
- Publication
International Journal of Laboratory Hematology, 2024, Vol 46, Issue 1, p141
- ISSN
1751-5521
- Publication type
Article
- DOI
10.1111/ijlh.14162