We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
ARH-77, an established human IgG-producing myeloma cell line. I. Morphology, B-cell phenotypic marker profile, and expression of Epstein-Barr virus.
- Authors
Drewinko, Benjamin; Mars, Wendy; Minowada, Jun; Burk, Kenneth H.; Trujillo, Jose M.; Drewinko, B; Mars, W; Minowada, J; Burk, K H; Trujillo, J M
- Abstract
Monolayer cultures of ARH-77 cells, a human myeloma cell line propagated in vitro, display a variety of morphologic entities ranging from small lymphocytes to classic plasma cells. The cells show intense pyronin and periodic acid-Schiff affinity but are negative for colloidal iron, sudan black, and naphtol AS-D chloroacetate esterase. The cells exhibit phenotypic markers pertaining to each stage of the B-cell lineage. They fail to display sheep erythrocyte and bovine erythrocyte-IgG antibody complex rosettes, common acute lymphocytic leukemia (ALL) antigens and T-cell antigens, but most cells display surface complement receptors, Ia-like antigens, and surface and intracytoplasmic Ig. Monoclonal antibodies were negative for T-antigens, myelomonocytic cell antigens, leukemia-associated antigens, and BA-1 and OKT-10 antigens. However, 100% of the cells were positive with OKT-9 and B3/25 antibodies that are specific for transferrin receptors. About 50% to 80% of the cells were positive for surface membrane immunoglobulin (kappa IgG) and about 10% to 50% for cytoplasmic immunoglobulin (kappa IgG). Virtually all cells were positive when tested for nuclear Epstein-Barr virus antigens.
- Publication
Cancer (0008543X), 1984, Vol 54, Issue 9, p1883
- ISSN
0008-543X
- Publication type
journal article
- DOI
10.1002/1097-0142(19841101)54:9<1883::AID-CNCR2820540919>3.0.CO;2-Q