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- Title
Protective effect of l-citrulline against acute gastric mucosal lesions induced by ischemia-reperfusion in rats.
- Authors
Gou, Lingshan; Zhang, Ling; Yin, Cui; Jia, Genguang; Yin, Xiaoxing; Zhuang, Xuemei; Xu, Xiaodong; Liu, Yi
- Abstract
The present study investigated the protective effect of l-citrulline on gastric mucosal injury induced by ischemia-reperfusion (IR) in rats. Under anesthesia, the celiac artery was clamped for 30 min, and then the clamp was removed for 60 min reperfusion. Sixty minutes before ischemia, l-citrulline was administered intragastrically at doses of 300, 600, and 900 mg/kg. After the experiment, the stomachs were removed for biochemical and histological examinations. Pretreatment with l-citrulline (300, 600, and 900 mg/kg) significantly ameliorated the gastric damage caused by IR. Moreover, l-citrulline prevented the production of lipid peroxidation and inhibited the increase of myeloperoxidase activity. The elevation in total nitric oxide synthase (NOS) activity, inducible NOS activity, and inducible NOS protein expression as well as the decrease in constitutive NOS activity and gastric mucus level in the gastric mucosa induced by IR were significantly prevented. However, the protective effect mediated by l-citrulline was significantly antagonized by coadministration of l-nitroarginine methyl ester (10 mg/kg, s.c.). These results suggest that part of the mechanism of gastric protection by l-citrulline might be through inhibiting neutrophil infiltration and preserving gastric mucus synthesis and secretion in rats, functions that are closely related to the maintenance of constitutive NOS activity.
- Subjects
DRUG efficacy; ORNITHINE carbamoyltransferase; GASTRIC mucosa; REPERFUSION injury; DRUG administration; DRUG dosage; NEUTROPHILS; LABORATORY rats; WOUNDS &; injuries
- Publication
Canadian Journal of Physiology & Pharmacology, 2011, Vol 89, Issue 5, p317
- ISSN
0008-4212
- Publication type
Article
- DOI
10.1139/y11-027