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- Title
Recruitment of SHP-1 Protein Tyrosine Phosphatase and Signalling by a Chimeric T-Cell Receptor–Killer Inhibitory Receptor.
- Authors
Christensen; Geisler
- Abstract
Receptors expressing the immunoreceptor tyrosine-based inhibitory motif (ITIM) in their cytoplasmic tail play an important role in the negative regulation of natural killer and B-cell activation. A subpopulation of T cells expresses the ITIM containing killer cell inhibitory receptor (KIR), which recognize MHC class I molecules. Following coligation of KIR with an activating receptor, the tyrosine in the ITIM is phosphorylated and the cytoplasmic protein tyrosine phosphatase SHP-1 is recruited to the ITIM via its SH2 domains. It is still not clear how SHP-1 affects T-cell receptor (TCR) signalling. In this study, we constructed a chimeric TCR–KIR receptor. We demonstrated that SHP-1 is recruited to the chimeric TCR–KIR receptor following T-cell stimulation with either anti-TCR monoclonal antibody (MoAb) or superantigen. However, in spite of this we could not detect any effect of SHP-1 on TCR signalling regarding total protein tyrosine phosphorylation, TCR down-regulation, mobilization of intracellular free calcium, or induction of the activation markers CD69 and CD25.
- Subjects
KILLER cells; MAJOR histocompatibility complex; PROTEIN-tyrosine phosphatase
- Publication
Scandinavian Journal of Immunology, 2000, Vol 51, Issue 6
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1046/j.1365-3083.2000.00727.x