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- Title
Ex Vivo Interferon-γ Response to Human Immunodefiency Virus-1 Derived Peptides in Human Immunodeficiency Virus-1 Infected Patients.
- Authors
Hober; Benyoucef; Chehadeh; De Groote; De La Tribonnière; Mouton; Wattré; Hober, Didier
- Abstract
The pattern of human immunodeficiency virus (HIV)-1 antigen-activated production of interferon (IFN)-γ by immunocompetent cells of HIV-1 infected patients has been studied using a simplified assay combining a small volume (25 μl) of whole blood stimulation with various HIV-1 antigens, and cytokine measurement in the same wells of microtitre plates (enzyme-linked immunotrapping assay, ELITA). The levels of IFN-γ were higher using this assay than in the supernatant from stimulated whole blood cultures, therefore ELITA was used in the rest of the study. Specific immune responses to HIV-1 proteins (gp120, p24) and synthetic peptides derived from these proteins and from gp41 were detected in patients, but not in healthy controls. Decreased levels of IFN-γ were observed in CDC class B (n = 5) and C (n = 4), compared with CDC class A (n = 5), following HIV-1 antigen-specific challenge. The positive response of cells from different patients to overlapping peptides of p25 (amino acids 329–344 and 335–351) was suggestive of a new epitope of HIV-1 gag recognized by T cells in the overlap region. In conclusion, the difference in in vitro antigen-specific T-cell responses of HIV-1-infected patients was shown using the ELITA method. Our results raise the possibility of using this method in screening specific antigens in HIV-1 infection.
- Subjects
INTERFERONS; PEPTIDES; HIV-positive persons
- Publication
Scandinavian Journal of Immunology, 2000, Vol 51, Issue 4, p429
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1046/j.1365-3083.2000.00707.x