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- Title
Monoclonal Antibodies Specific for Variable and Constant Domains of Murine λ Chains.
- Authors
Bogen, B.
- Abstract
Rat monoclonal antibodies directed against the BALB/c myeloma protein M315 (α.λ2) are described. 9A8 (IgG1) binds the V domain of λ2 and cross-reacts with λ1 and λ3 chains. 2B6 (IgG2a) is directed to the C domain of λ2 and cross-reacts with Cλ3. Th antibodies bind isolated chains as well as complete immunoglobulins. The monoclonals detect soluble immunoglobulin (radioimmunoassay), immunoglobulin immobilized on polystyrene (enzyme-linked immunosorbent assay), immunoglobulin bound to nitrocellulose), and surface immunoglobulin intercalated in cell membranes (immunofluorescence). The antibodies are easily purified on protein G immunosorbents and may be biotinylated or conjugated with fluorescein isothiocyanate without loss of capacity to bind. In addition to the anti-λ antibodies, a Cα2/Cα3-specific monoclonal antibody, 8D2 (IgG2a) is described.
- Subjects
IMMUNOGLOBULINS; MONOCLONAL antibodies; TUMOR proteins; PLASMA cells; BLOOD proteins
- Publication
Scandinavian Journal of Immunology, 1989, Vol 29, Issue 3, p273
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1989.tb01125.x