We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Reverse transcription recombinase-aided amplification assay for H5 subtype avian influenza virus.
- Authors
Wang, Suchun; Li, Yang; Zhang, Fuyou; Jiang, Nan; Zhuang, Qingye; Hou, Guangyu; Jiang, Lijian; Yu, Jianmin; Yu, Xiaohui; Liu, Hualei; Zhao, Chenglong; Yuan, Liping; Huang, Baoxu; Wang, Kaicheng
- Abstract
Background: The H5 subtype avian influenza virus (AIV) has caused huge economic losses to the poultry industry and is a threat to human health. A rapid and simple test is needed to confirm infection in suspected cases during disease outbreaks. Methods: In this study, we developed a reverse transcription recombinase-aided amplification (RT-RAA) assay for the detection of H5 subtype AIV. Assays were performed at a single temperature (39 °C), and the results were obtained within 20 min. Results: The assay showed no cross-detection with Newcastle disease virus or infectious bronchitis virus. The analytical sensitivity was 103 RNA copies/μL at a 95% confidence interval according to probit regression analysis, with 100% specificity. Compared with published reverse transcription quantitative real-time polymerase chain reaction assays, the κ value of the RT-RAA assay in 420 avian clinical samples was 0.983 (p < 0.001). The sensitivity for avian clinical sample detection was 97.26% (95% CI, 89.56–99.52%), and the specificity was 100% (95% CI, 98.64–100%). Conclusions: These results indicated that our RT-RAA assay may be a valuable tool for detecting H5 subtype AIV.
- Subjects
AVIAN influenza A virus; AVIAN infectious bronchitis virus; NEWCASTLE disease virus; PROBIT analysis; POLYMERASE chain reaction
- Publication
Virology Journal, 2022, Vol 19, Issue 1, p1
- ISSN
1743-422X
- Publication type
Article
- DOI
10.1186/s12985-022-01807-0