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- Title
Regeneração de plantas a partir de dois tipos de explantes de milho submetidos à transformação genética por biobalística.
- Authors
Andrade Vidigal, Tiago Mateus; Schuster, Ivan; Texeira, Leandra Regina; Barros Colauto, Nelson
- Abstract
The use of biolistic on genetic transformation of plants requires an optimization of several parameters, including the protocol development for plant regeneration from transformed cells. The objective of this research was to evaluate the regeneration efficiency of maize plants from embryogenic callus and explants from split seeds submitted to transformation by biolistic using the gene bar as a selectable marker. It was submitted 2000 clusters of embryogenic callus and 2000 divided maize seeds to transformation by biolistic. The selection of transformation events was in culture medium supplemented with ammonium glifosinate. Selected seedlings were acclimatized and transplanted to pots in a greenhouse. Using somatic embryogenesis, the regeneration efficiency was 0.2%, while using split seeds explants the regeneration efficiency was 1.3%. Although both protocols allow the maize plant regeneration after transformation by biolistic, there is a need to increase its efficiency.
- Publication
Ciência Rural, 2014, Vol 44, Issue 10, p1804
- ISSN
0103-8478
- Publication type
Article
- DOI
10.1590/0103-8478cr20130803