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- Title
Transplante autólogo de células monononucleares da medula óssea em úlcera de córnea experimental em cães.
- Authors
Tognoli, Guilherme Kanciukaitis; Débora Cristina Olsson; Martins, Danieli Brolo; Júnior, Eduardo de Bastos Santos; Fabiano Zanini Salbego; de Oliveira, Graziela Kopinits; Braga, Fabrício de Vargas Arigony; Raiser, Alceu Gaspar; Dezengrini, Renata; Ferreira da Cruz, Fernando Silvério; de Castro, Márcio Botelho; Rosa, Mariana Carvalho; Carregaro, Adriano Bonfim; Pippi, Ney Luis
- Abstract
Bone marrow (BM) mononuclear cells (MC) are a great subject in tissual regeneration. The main stem cell source to the eye is the limbus. Theses cells replace injured corneal cells, however, if the limbal stem cells are not functional, a limbal deficiency with concomitant conjunctivalization takes place. This pathological condition can be caused for several reasons, in which alkali burns are the most common. To conduct a research about transplanted BM MC presence, the cells homing and to histopathologically compare the treated and sham group, an experimental corneal ulcer model associated with MC autotransplant was used. Sixteen, male or female, stray dogs suffered experimental corneal ulceration with sodium hydroxide soaked filter discs. After the lesions, animals were submitted to subconjunctival autotransplant of previously marked BM MC. The evaluation was made by immunofluorescence on the sixth day after lesions creation and histopathology was conducted 15 days after the same procedure, when it was possible to observe that the MC grafted in the injured area, the cells did not execute the homing process and, despite the inflammatory decrease, they did not help the corneal epithelial healing process in a short term evaluation. Thus, future studies about MC transplantations in corneal ulcers are indicated.
- Subjects
BONE marrow; HEMATOPOIETIC system; DOGS; EXPERIMENTS; RESEARCH methodology; HISTOPATHOLOGY; HEMATOPOIESIS
- Publication
Ciência Rural, 2009, Vol 39, Issue 1, p148
- ISSN
0103-8478
- Publication type
Article