We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Expression of the immune checkpoint receptor TIGIT in Hodgkin's lymphoma.
- Authors
Li, Wenchao; Blessin, Niclas C.; Simon, Ronald; Kluth, Martina; Fischer, Kristine; Hube-Magg, Claudia; Makrypidi-Fraune, Georgia; Wellge, Björn; Mandelkow, Tim; Debatin, Nicolaus F.; Pott, Laura; Höflmayer, Doris; Lennartz, Maximilian; Sauter, Guido; Izbicki, Jakob R.; Minner, Sarah; Büscheck, Franziska; Uhlig, Ria; Dum, David; Krech, Till
- Abstract
Hodgkin's lymphoma (HL) is characterized by a high background of inflammatory cells which play an important role for the pathogenesis of the disease. T cell immunoreceptor with Ig and ITIM domains (TIGIT) is an inhibitory immune checkpoint receptor and a putative target for novel immunotherapies. To study patterns of TIGIT expression in the T cell background surrounding malignant cells including Hodgkin cells, Reed-Sternberg cells and histiocytic cells, a microenvironment (ME) tissue microarray (TMA) was constructed from tissue punches measuring 2 mm in diameter obtained from formalin-fixed tissue samples of Hodgkin's lymphoma lymph nodes (n = 40) and normal human tonsil (n = 2). The ME-TMA was stained by brightfield and fluorescence multiplex immunohistochemistry (IHC) to evaluate expression levels of TIGIT and PD-1 as well as standard lymphocyte markers (CD3, CD8, CD4, FOXP3) in the lymphocytic background. All analyzed cases of HL contained 9-99% (median: 86%) of TIGIT+ lymphoid cells. In general, TIGIT localized to the same cells as PD-1. Strikingly, expression levels of TIGIT and PD-1 were highly variable among the analyzed samples. Highest levels of TIGIT and PD-1 were found in one sample of nodular lymphocytic-predominant HL (NLPHL). In conclusion, TIGIT expression is highly variable between patients with Hodgkin's lymphoma. Our results encourage further studies evaluating the role of TIGIT as a target for immunotherapies in Hodgkin's lymphoma.
- Subjects
HODGKIN'S disease; T cell receptors; HODGKIN'S disease treatment; IMMUNOTHERAPY; IMMUNOHISTOCHEMISTRY; CELL physiology; CELL receptors; GENES; T cells
- Publication
BMC Cancer, 2018, Vol 18, Issue 1, p1
- ISSN
1471-2407
- Publication type
journal article
- DOI
10.1186/s12885-018-5111-1