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- Title
Effect of Two Lipid Emulsions on Reversing High-Dose Levobupivacaine-Induced Reduced Vasoconstriction in the Rat Aortas.
- Authors
Ok, Seong-Ho; Park, Chang-Shin; Kim, Hye Jung; Lee, Soo Hee; Choi, Bo-Hwa; Eun, So Young; Kim, Kyung-Nam; Yang, Seong Min; Shin, Il-Woo; Choi, Mun-Jeoung; Sohn, Ju-Tae
- Abstract
The goals of this study were to determine which lipid emulsion (Intralipid ® and Lipofundin MCT/LCT ®) is more effective in reversing high-dose levobupivacaine-induced reduced vasoconstriction in isolated rat aortas and to examine the associated cellular mechanisms with a particular focus on the endothelium. Two lipid emulsion concentration–response curves were generated using high-dose levobupivacaine-induced reduced vasoconstriction and vasodilation of isolated aortas pretreated with or without 60 mM KCl. Endothelial nitric oxide synthase (eNOS) and caveolin-1 phosphorylation were measured in rat aortic tissue treated with levobupivacaine in the presence or absence of lipid emulsion. Dichlorofluorescein oxidation, a measure of reactive oxygen species production, was measured in lipid emulsion-treated human umbilical vein endothelial cells. In levobupivacaine (0.3 mM)-induced reduced vasoconstriction of isolated aorta, the magnitude of the Intralipid ®- and Lipofundin MCT/LCT ®-mediated reversal was not significantly different. Lipid emulsion reversal of levobupivacaine-induced reduced vasoconstriction was greater in endothelium-intact aortas than in endothelium-denuded aortas. The two lipid emulsions similarly inhibited levobupivacaine-induced eNOS phosphorylation in aortic tissue. Pretreatment with both lipid emulsions increased dichlorofluorescein oxidation. Both Intralipid ® and Lipofundin MCT/LCT ® are equally effective for vascular tone recovery from high-dose levobupivacaine-induced reduced vasoconstriction. This reversal is mediated partially by decreasing nitric oxide bioavailability.
- Subjects
LIPIDS; EMULSIONS (Pharmacy); DRUG dosage; ANESTHETICS; VASOCONSTRICTION; LABORATORY rats; AORTA abnormalities
- Publication
Cardiovascular Toxicology, 2013, Vol 13, Issue 4, p370
- ISSN
1530-7905
- Publication type
Article
- DOI
10.1007/s12012-013-9218-y