We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Live Monitoring of Strain‐Promoted Azide Alkyne Cycloadditions in Complex Reaction Environments by Inline ATR‐IR Spectroscopy.
- Authors
Svatunek, Dennis; Eilenberger, Gottfried; Denk, Christoph; Lumpi, Daniel; Hametner, Christian; Allmaier, Günter; Mikula, Hannes
- Abstract
The strain‐promoted azide alkyne cycloaddition (SPAAC) is a powerful tool for forming covalent bonds between molecules even under physiological conditions, and therefore found broad application in fields ranging from biological chemistry and biomedical research to materials sciences. For many applications, knowledge about reaction kinetics of these ligations is of utmost importance. Kinetics are commonly assessed and studied by NMR measurements. However, these experiments are limited in terms of temperature and restricted to deuterated solvents. By using an inline ATR‐IR probe we show that the cycloaddition of azides and alkynes can be monitored in aqueous and even complex biological fluids enabling the investigation of reaction kinetics in various solvents and even human blood plasma under controlled conditions in low reaction volumes.
- Subjects
MATERIALS science; BIOCHEMISTRY; COMPLEX fluids; CHEMICAL kinetics; SPECTROMETRY; PAUSON-Khand reaction
- Publication
Chemistry - A European Journal, 2020, Vol 26, Issue 44, p9851
- ISSN
0947-6539
- Publication type
Article
- DOI
10.1002/chem.201905478