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- Title
Methylation profiling of biosynthetic genes reveals the role of D-galacturonic acid reductase in ascorbic acid accumulation in tomato fruit.
- Authors
Wang, Yaru; Wang, Ying; Li, Fangman; Shang, Lele; Tao, Jinbao; Zhang, Xingyu; Ren, Zongjun; Dong, Haiqiang; Gai, Wenxian; Zhang, Yuyang
- Abstract
Ascorbic acid (AsA) is an important nutrient that contributes to the primary flavour of tomato fruits and human health. While the regulation of the transcription of AsA biosynthetic genes has been well demonstrated, the epigenetic alteration underlying AsA accumulation remains uncertain. In this study, a methyltransferase inhibitor (5-azacytidine) was applied to immature tomato fruit with a methyltransferase inhibitor (5-azacytidine) and effects on AsA accumulation were identified. Inhibiting DNA methylation increased the accumulation of AsA in tomato leaves and fruit. We also isolated a biosynthetic AsA gene, SlGalUR5, which encodes D-galacturonic acid reductase. SlGalUR5 showed a decrease in DNA methylation levels and higher transcription levels in the Slmet1 mutant while exhibiting inverted tendencies in the Sldml2 mutant. Treatment with 5-azacytidine dramatically reduced the DNA methylation levels of SlGalUR5 in fruit. In contrast, transcription profiles of SlGalUR5 and enzyme activity were improved in 5-azacytidine-treated fruit. Our finding reveals new insight into the epigenomic modification of SlGalUR5 involved in ascorbic acid accumulation and provides a potential means of increasing AsA levels for tomato breeding.
- Publication
Plant Growth Regulation, 2022, Vol 98, Issue 2, p281
- ISSN
0167-6903
- Publication type
Article
- DOI
10.1007/s10725-022-00863-4