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- Title
Characterization of a novel hydroxynitrile lyase from Nandina domestica Thunb.
- Authors
Isobe, Kimiyasu; Kitagawa, Asuka; Kanamori, Kazuma; Kashiwagi, Nozomi; Matsui, Daisuke; Yamaguchi, Takuya; Fuhshuku, Ken-ichi; Semba, Hisashi; Asano, Yasuhisa
- Abstract
The leaves of Nandina domestica Thunb. exhibited high hydroxynitrile lyase (HNL) activity in (R)-mandelonitrile synthesis. The specific activity of young leaves was significantly higher than that of mature leaves. We isolated two HNLs with molecular mass of 24.9 kDa (NdHNL-S) and 28.0 kDa (NdHNL-L) from the young leaves. Both NdHNLs were composed of two identical subunits, without FAD and carbohydrates. We purified NdHNL-L and revealed its enzymatic properties. The whole deduced amino acid sequence of NdHNL-L was not homologous to any other HNLs, and the specific activity for mandelonitrile synthesis by NdHNL-L was higher than that by other plant HNLs. The enzyme catalyzed enantioselective synthesis of (R)-cyanohydrins, exhibited high activity at pH 4.0, and high stability in the pH range of 3.5-8.0 and below 55°C. Thus, NdHNL-L is a novel HNL with novel amino acid sequence and has a potential for the efficient production of (R)-cyanohydrins.
- Subjects
LYASES; HEAVENLY bamboo; NITRILE synthesis
- Publication
Bioscience, Biotechnology & Biochemistry, 2018, Vol 82, Issue 10, p1760
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1080/09168451.2018.1490171