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- Title
A label-free electrochemical biosensor based on tubulin immobilized on gold nanoparticle/glassy carbon electrode for the determination of vinblastine.
- Authors
Haghshenas, Esmaeel; Madrakian, Tayyebeh; Afkhami, Abbas; Saify Nabiabad, Haidar
- Abstract
Vinblastine (VLB) is prescribed for a wide variety of cancers. Therefore, development of sensitive methods for early diagnosis is urgently required. In this work, a highly sensitive and label-free impedimetric biosensor was fabricated for the electrochemical detection of VLB. First, the gold nanoparticles (AuNPs) were electrodeposited on the surface of a glassy carbon electrode (GCE). 3-Mercaptopropionic acid (MPA) was self-assembled over the AuNPs. Then, tubulin (TUB), as a receptor, was covalently immobilized at the AuNPs/GCE surface via carbodiimide coupling reaction using N-(3 dimethylaminopropyl)- N′-ethyl carbodiimide (EDC) and N-hydroxy succinimide (NHS). The step-by-step modification process was characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) in the presence of a redox probe [Fe(CN)]. The VLB concentration was measured through the increase of impedance values in the corresponding specific binding of VLB and TUB. The increased electron-transfer resistance ( R ) values were proportional to the value of VLB concentrations in the range of 0.4 to 65.0 nmol L with a detection limit of 8.4 × 10 nmol L (SN = 3). The practical analytical performance of the proposed method was demonstrated by determination of VLB in plant extracts and human serum samples with satisfactory recoveries.
- Subjects
BIOSENSORS testing; NATIVE element minerals; CANCER diagnosis; CANCER treatment; CYCLIC voltammetry; IMPEDANCE spectroscopy
- Publication
Analytical & Bioanalytical Chemistry, 2017, Vol 409, Issue 22, p5269
- ISSN
1618-2642
- Publication type
Article
- DOI
10.1007/s00216-017-0471-y