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- Title
Dynamic analysis of pathogen-infected host cells using quantitative phase microscopy.
- Authors
Lee, Seungrag; Kim, Young Ran; Lee, Ji Yong; Rhee, Joon Haeng; Park, Chang-Soo; Kim, Dug Young
- Abstract
We present the real-time quantitative analysis of Vibrio vulnificus–infected host cells using quantitative phase microscopy (QPM) based on interferometric techniques. This provides the ability to retrieve the phase or optical path-length distribution over the cell with nanometer path-length sensitivity from a single interferogram image. We have used QPM to study dynamic cell morphologic changes and to noninvasively quantify the cell volumes of rat basophilic leukemia RBL-2H3 cells infected with V. vulnificusstrains: wild type (MO6–24O) and RtxA1 toxin mutant (CMM770). During the process of V. vulnificusinfection in RBL-2H3 cells, the dynamic changes of quantitative phase images, cell volumes, and areas were observed in real time using QPM. In contrast, dramatic changes were not detected in RBL-2H3 cells infected with the noncytotoxic RtxA1 toxin mutant. The results showed good correlation between QPM analysis and biochemical assays, such as lactate dehydrogenase assay or -hexosaminidase release assay. We suggest that QPM is a powerful quantitative method to study the dynamic process of host cells infected with pathogens in a noninvasive manner.
- Subjects
HOST-parasite relationships; PHASE-contrast microscopes; STEREOLOGY; INTERFEROMETRY; NANOSTRUCTURED materials; REAL-time computing; VIBRIO vulnificus; BACTERIAL diseases
- Publication
Journal of Biomedical Optics, 2011, Vol 16, Issue 3, p036004
- ISSN
1083-3668
- Publication type
Article
- DOI
10.1117/1.3548882