We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Staurosporine-induced apoptosis is independent of p16 and p21 and achieved via arrest at G2/M and at G1 in U251MG human glioma cell line.
- Authors
Yamasaki, Fumiyuki; Hama, Seiji; Yoshioka, Hiroyuki; Kajiwara, Yoshinori; Yahara, Kaita; Sugiyama, Kazuhiko; Heike, Yuji; Arita, Kazunori; Kurisu, Kaoru
- Abstract
Objective. The mechanisms involved in the cell cycle and cell death remain unresolved despite much investigation. Staurosporine induces cell death and G1 or G2/M arrest in a dose-dependent manner, but the mechanisms remain unknown. Methods. In the present study an adenovirus vector expressing p16 or p21 genes in human glioma cell lines was used to examine cell cycle regulation and cell death induced by staurosporine. Results. A low concentration (≤10 nM) of staurosporine induced G1 arrest of U251MG cells, whereas a high concentration (≥30 nM) induced G2/M arrest and finally induced apoptosis via a caspase-3-activated pathway from both the G2/M and G1 phases. However, pRb was dephosphorylated and cdc2 was inhibited at both the low and the high concentrations of staurosporine, indicating that the mechanisms of cell cycle regulation are not simply p53-Rb- or cdc2-dependent pathways. Conclusions. Forced G1 arrest by transfection with p16 or p21 genes did not alter the rate of staurosporine-induced cell death. This implies that an unknown pathway of apoptosis occurs from the G1 phase.
- Subjects
GLIOMAS; APOPTOSIS; CELL death; CELL lines
- Publication
Cancer Chemotherapy & Pharmacology, 2003, Vol 51, Issue 4, p271
- ISSN
0344-5704
- Publication type
Article
- DOI
10.1007/s00280-002-0562-z